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Huiquan Wu, Erik Read, Maury White, Brittany Chavez, Kurt Brorson, Cyrus Agarabi, Mansoor Khan
《化学科学与工程前沿(英文)》 2015年 第9卷 第3期 页码 386-406 doi: 10.1007/s11705-015-1533-3
关键词: process analytical technology (PAT) Fourier-transform infrared (FTIR) spectroscopy partial least squares (PLS) regression mouse IgG3 bioreactor cell culture process real time process monitoring
《环境科学与工程前沿(英文)》 doi: 10.1007/s11783-021-1483-6
While a growing number of wastewater treatment plants (WWTPs) are being retrofitted from the conventional activated sludge (CAS) process to the membrane bioreactor (MBR) process, the debate on the techno-economy of MBR vs. CAS has continued and calls for a thorough assessment based on techno-economic valuation.
关键词: Membrane bioreactor (MBR) Conventional activated sludge (CAS) Cost-benefit analysis Data envelopment analysis Net profit
Positive stool culture could predict the clinical outcomes of haploidentical hematopoietic stem cell
Lijuan Hu, Qi Wang, Xiaohui Zhang, Lanping Xu, Yu Wang, Chenhua Yan, Huan Chen, Yuhong Chen, Kaiyan Liu, Hui Wang, Xiaojun Huang, Xiaodong Mo
《医学前沿(英文)》 2019年 第13卷 第4期 页码 492-503 doi: 10.1007/s11684-019-0681-0
关键词: haploidentical hematopoietic stem cell transplantation stool culture Candida
Microfluidics for cell-cell interactions: A review
Rui Li,Xuefei Lv,Xingjian Zhang,Omer Saeed,Yulin Deng
《化学科学与工程前沿(英文)》 2016年 第10卷 第1期 页码 90-98 doi: 10.1007/s11705-015-1550-2
关键词: microfluidic chip co-culture cell-cell interactions review
Performance of bioferric-submerged membrane bioreactor for dyeing wastewater treatment
ZOU Haiyan, XI Danli
《环境科学与工程前沿(英文)》 2007年 第1卷 第3期 页码 374-380 doi: 10.1007/s11783-007-0064-7
Vo-Van Giau, Yoon-Hee Park, Kyu-Hwan Shim, Sang-Wook Son, Seong-Soo A. An
《化学科学与工程前沿(英文)》 2019年 第13卷 第1期 页码 90-97 doi: 10.1007/s11705-018-1766-z
The potential applications of nanomaterials used in nanomedicine as ingredients in drug delivery systems and in other products continue to expand. When nanomaterials are introduced into physiological environments and driven by energetics, they readily associate proteins forming a protein corona (PC) on their surface. This PC could result in an alteration of the nanomaterial’s surface characteristics, affecting their interaction with cells due to conformational changes in adsorbed protein molecules. However, our current understanding of nanobiological interactions is still very limited. Utilizing a liquid chromatography–mass spectroscopy/mass spectroscopy technology and a Cytoscape plugin (ClueGO) approach, we examined the composition of the PC for a set of zinc oxide nanoparticles (ZnONP) from cell culture media typically and further analyzed the biological interaction of identified proteins, respectively. In total, 36 and 33 common proteins were investigated as being bound to ZnONP at 5 min and 60 min, respectively. These proteins were further analyzed with ClueGO, a Cytoscape plugin, which provided gene ontology and the biological interaction processes of identified proteins. Proteins bound to the surface of nanoparticles that may modify the structure, therefore the function of the adsorbed protein could be consequently affect the complicated biological processes.
基于液滴微流控的分级结构反蛋白石多孔支架用于仿生三维细胞共培养 Article
邵长敏, 刘羽霄, 池俊杰, 叶方富, 赵远锦
《工程(英文)》 2021年 第7卷 第12期 页码 1778-1785 doi: 10.1016/j.eng.2020.06.031
三维(3D)细胞培养具有更好地模拟天然组织特异性的优势,在药物开发、毒性测试和组织工程中发挥着重要作用。然而,现有的3D细胞培养的支架或微载体通常尺寸有限,并且在模拟生物体内血管复合体方面表现不佳。因此,本研究提出了一种通过简单的微流控方法制备的新型分级结构反蛋白石多孔支架,用于促进3D细胞共培养。该支架是基于微流控乳液液滴模板和惰性聚合物聚合的复合概念构建的。研究结果表明,该支架能够保证细胞培养过程中的营养供给,从而实现大面积的细胞培养。此外,通过在该支架中连续种植不同的细胞,本文还开发了内皮细胞包裹肝细胞的3D细胞共培养系统,用于构建功能化组织。研究结果表明,该支架用于细胞共培养系统,有助于维持肝细胞特定的体内功能。该分级结构反蛋白石多孔支架为3D细胞培养甚至仿生组织的构建奠定了基础。
Ruining WANG,Yubao ZHI,Junqing GUO,Qingmei LI,Li WANG,Jifei YANG,Qianyue JIN,Yinbiao WANG,Yanyan YANG,Guangxu XING,Songlin QIAO,Mengmeng ZHAO,Ruiguang DENG,Gaiping ZHANG
《农业科学与工程前沿(英文)》 2015年 第2卷 第3期 页码 230-236 doi: 10.15302/J-FASE-2015071
关键词: classical swine fever virus virus purification tangential-flow filtration size-exclusion chromatography
《环境科学与工程前沿(英文)》 2021年 第15卷 第6期 doi: 10.1007/s11783-021-1409-3
• MFC promoted the nitrogen removal of anammox with Fe-C micro-electrolysis.
《环境科学与工程前沿(英文)》 2023年 第17卷 第6期 doi: 10.1007/s11783-023-1668-2
● Efficient carbon methanation and nitrogen removal was achieved in AnMBR-PN/A system.
关键词: Anaerobic membrane bioreactor Partial nitrification/Anammox Carbon separation Chemolitrophic nitrogen removal
发酵过程多水平问题及其生物反应器装置技术研究——基于过程参数相关的发酵过程优化与放大技术
张嗣良
《中国工程科学》 2001年 第3卷 第8期 页码 37-45
回顾了发酵过程优化与放大所依据的基本思想和方法,认为采用以动力学为基础的最佳工艺控制点为依据的静态操作方法,实质上只是化学工程宏观动力学概念在发酵工程上的延伸,往往忽视细胞代谢流的存在。以细胞代谢流的分析与控制为核心的生物反应工程学的观点,通过实验研究,提出了基于参数相关的发酵过程多水平问题研究的优化技术和发酵过程多参数调整的放大技术。随着过程传感技术和计算机技术的发展,设计了一种定型为FUS-50L(A)的新概念生物反应器。这种新型生物反应器是以物料流检测为手段,过程优化与放大为目标,成功地应用在青霉素、红霉素、金霉素、肌苷、鸟苷发酵和Pichia酵母表达系统的基因工程人血清白蛋白(rh-SA)、疟疾疫苗等高密度高表达培养,大幅度提高发酵水平,并直接放大到几百升,甚至100 m3以上生产规模发酵罐。
关欣,周景文,堵国成,陈坚
《中国工程科学》 2023年 第25卷 第3期 页码 251-262 doi: 10.15302/J-SSCAE-2023.07.018
细胞培养肉是融合先进生物技术、食品技术的战略性新兴产业,未来有可能带动农业领域的产业结构调整和发展模式转变;细胞培养肉技术正处于向商品化迈进的阶段,开展相关产业技术发展态势分析具有价值。本文梳理了细胞培养肉生产核心原料和试剂的产业态势及研究进展,涵盖良种动物、种子细胞、培养基、支架材料;分析了支持细胞培养肉生产的基础装备设施(生物安全柜、生物反应器及其他常用装备)制造技术瓶颈与行业竞争格局;剖析了细胞培养肉规模化生产的核心技术体系及发展动态,涉及种子细胞库建立、低成本培养基研制、大规模培养工艺、食品化加工技术。针对我国细胞培养肉产业基础的薄弱之处,提出了针对性发展建议:加快试剂装备国产化进程、完善细胞培养肉产业链,完善培养肉技术体系、加强知识产权国际布局,优化学科专业布局、校企融合双元育人,完善产品风险评估、论证和发布团体标准,加强科学知识普及、提高公众接受度。
Key stakeholders’ perspectives on the ideal partnering culture in construction projects
Gunnar J. LÜHR, Marian G. C. BOSCH-REKVELDT, Mladen RADUJKOVIC
《工程管理前沿(英文)》 2022年 第9卷 第2期 页码 312-325 doi: 10.1007/s42524-020-0135-z
关键词: project culture organisational culture partnering construction culture stakeholder perspectives German
Preliminary study of groundwater denitrification using a composite membrane bioreactor
Lihui ZHANG, Guomin CAO, Yulei FEI, Hong DING, Mei SHENG, Yongdi LIU
《环境科学与工程前沿(英文)》 2011年 第5卷 第4期 页码 604-609 doi: 10.1007/s11783-011-0274-x
关键词: groundwater nitrate denitrification composite membrane bioreactor (CMBR) immobilized cell membrane
标题 作者 时间 类型 操作
Real time monitoring of bioreactor mAb IgG3 cell culture process dynamics via Fourier transform infraredspectroscopy: Implications for enabling cell culture process analytical technology?
Huiquan Wu, Erik Read, Maury White, Brittany Chavez, Kurt Brorson, Cyrus Agarabi, Mansoor Khan
期刊论文
Techno-economic characteristics of wastewater treatment plants retrofitted from the conventional activated sludge processto the membrane bioreactor process
期刊论文
Positive stool culture could predict the clinical outcomes of haploidentical hematopoietic stem cell
Lijuan Hu, Qi Wang, Xiaohui Zhang, Lanping Xu, Yu Wang, Chenhua Yan, Huan Chen, Yuhong Chen, Kaiyan Liu, Hui Wang, Xiaojun Huang, Xiaodong Mo
期刊论文
Microfluidics for cell-cell interactions: A review
Rui Li,Xuefei Lv,Xingjian Zhang,Omer Saeed,Yulin Deng
期刊论文
Performance of bioferric-submerged membrane bioreactor for dyeing wastewater treatment
ZOU Haiyan, XI Danli
期刊论文
Dynamic changes of protein corona compositions on the surface of zinc oxide nanoparticle in cell culture
Vo-Van Giau, Yoon-Hee Park, Kyu-Hwan Shim, Sang-Wook Son, Seong-Soo A. An
期刊论文
Efficient purification of cell culture-derived classical swine fever virus by ultrafiltration and size-exclusion
Ruining WANG,Yubao ZHI,Junqing GUO,Qingmei LI,Li WANG,Jifei YANG,Qianyue JIN,Yinbiao WANG,Yanyan YANG,Guangxu XING,Songlin QIAO,Mengmeng ZHAO,Ruiguang DENG,Gaiping ZHANG
期刊论文
Reutilize tire in microbial fuel cell for enhancing the nitrogen removal of the anammox process coupled
期刊论文
Methanation and chemolitrophic nitrogen removal by an anaerobic membrane bioreactor coupled partial nitrification
期刊论文
Key stakeholders’ perspectives on the ideal partnering culture in construction projects
Gunnar J. LÜHR, Marian G. C. BOSCH-REKVELDT, Mladen RADUJKOVIC
期刊论文